Part:BBa_K4630101:Experience
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Applications of BBa_K4630101
We applied stgRNA barcode cassette 2 to connect after stgRNA barcode cassette 1 in order to verify the feasibility of multi-level knockout records. In addition, stgRNA barcode cassette 2 serves as a building block to provide a template for the subsequent design of stgRNA barcode cassette N. The subsequent stgRNA barcode cassette N only needs to change the barcode sequence and stgRNA sequence of stgRNA barcode cassette 2 to become a new-level editing and recording module
Gel electrophoresis map of plasmid
Figure. 1 Gel electrophoresis map of stgRNA-barcode-cassette 2
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